The service said it had received 27 bids, far fewer than the 45 bidders and 63 bids it received in its first Bitcoin auction in June. The winner, or winners, of the second auction will be notified on Friday by 5 p.m. Eastern time.

The Silk Road site was shut down in October 2013 after the authorities said it was a marketplace for illegal drugs and other illicit activities. In the auction five months ago, the Marshals Service sold nearly 30,000 Bitcoins.

The agency declined to comment further on Thursday.

Some of the same investors who participated in the first auction again submitted bids this time. SecondMarket and Pantera Bitcoin both allowed customers to bid through syndicates. The venture capitalist Timothy C. Draper, who was the first auction’s sole winner, said he planned to participate in Thursday’s auction.

But some bidders in the first auction chose to sit out the latest one. The Bitcoin Shop did not participate, nor did Rangeley Capital. Alex Waters, chief executive and co-founder of Coin Apex, who appeared to send his bid live on Bloomberg Television during the first auction, was also absent this time. Coinbase, a Bitcoin payment processor that participated in the first auction, declined to comment.

The results of the Marshals Service’s first auction may have dissuaded bidders from participating in Thursday’s auction, said Gil Luria, an analyst with Wedbush Securities. “The big difference is that people already saw how last time went.”

Though the Marshals Service did not announce the price of Mr. Draper’s winning bid, it is widely assumed among Bitcoin investors that he bid above Bitcoin’s market price at the time. As a result, some investors who had hoped to get a bargain last time saw little point in participating in this auction. Bitcoin is now trading at around $370, down from about $570 at the start of the June auction.

“Unless you can get a steep discount to market, it’s a lot of risk,” said Charles Allen, the chief executive of the Bitcoin Shop. “If we’re not going to get a discount, what’s the point?”

Winklevoss Capital, a start-up venture firm started by Cameron and Tyler Winklevoss, the twin brothers known for their legal battles with the Facebook co-founder Mark Zuckerberg, did not participate in either auction. “We don’t generally participate in auctions where there is limited information, a high likelihood of a winner’s curse and a low likelihood of achieving an efficient price. That being said, anything can happen and we wish the participants the best of luck,” Cameron Winklevoss said in a statement on Thursday.

The latest auction is for Bitcoins found on computer hardware belonging to Ross Ulbricht, who is accused of creating Silk Road. Prosecutors and Mr. Ulbricht agreed in January that the government could sell the virtual currency it had seized.

The government’s two auctions represent only a fraction of the total amount of Bitcoins seized in connection with Silk Road. Including 144,336 Bitcoins found on computer hardware belonging to Mr. Ulbricht, the government says it has recovered 173,991 Bitcoins. A spokeswoman for the Marshals Service, Lynzey Donahue, said in an email when the second auction was announced that the agency anticipated selling the remaining Bitcoins, but that “no exact dates have been determined.”

(Published by Deal Book – December 4, 2014)